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abf2 [2019-07-08 11:35:40]
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abf2 [2019-07-08 11:35:40]

alpha-L-arabinofuranosidase
Locus
BSU28510
Isoelectric point
5.33
Molecular weight
56.34 kDa
Protein length
495 aa Sequence Blast
Gene length
1488 bp Sequence Blast
Function
arabinan degradation
Product
alpha-L-arabinofuranosidase
Essential
no
E.C.
3.2.1.55
Synonyms
xsa
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of arabinan/ arabinose/ arabitol

    • Gene

    Coordinates
    2,913,661 2,915,148

    The protein

    Catalyzed reaction/ biological activity

  • Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to Swiss-Prot)

    • Protein family

  • glycosyl hydrolase 51 family (according to Swiss-Prot)

    • Structure

  • 2VRQ (from Thermobacillus xylanilyticus, 66% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    abf2
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AraR: repression, PubMed, in AraR regulon

    • Regulation

  • induced by arabinose (AraR) PubMed

    • Additional information

  • A ncRNA is predicted between 'trxA' and 'abf2' PubMed
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    Biological materials

    Mutant

  • BKE28510 (abf2::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCTCTGATCATTCCTT, downstream forward: _UP4_TAATCGCGCAAGAGCGCCGG
  • BKK28510 (abf2::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCTCTGATCATTCCTT, downstream forward: _UP4_TAATCGCGCAAGAGCGCCGG

    • References

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